The Pharm Eur (1) instruction on preparing the inoculum for the AET states: Although this seems to be a minor point, it does serve to illustrate some of the inherent difficulties in microbiological testing and the need for experienced and academically trained microbiologists to head the laboratory. The compendial antimicrobial efficacy test (AET) requires inoculation of the product with microorganisms to a final concentration of approximately 106 CFU/mL. This essay describes the use of turbidity to estimate microbial concentration in a suspension, using the Antimicrobial Efficacy Test as the example. If the inoculum concentration is determined by plating, the inoculum is several days old before use. It appears here with permission.Ī common issue for the microbiology lab is the determination of starting inoculum concentration. This article first appeared in the PMF Newsletter of August, 2006 and is protected by copyright to PMF. ![]() Measurement of Cell Concentration in Suspension by Optical Density
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